Wine polyphenol resveratrol inhibits contractions of isolated rat uterus by activation of smooth muscle inwardly rectifying potassium channels
2016
Novaković, Radmila (Faculty of Medicine, Belgrade (Serbia). Institute of Pharmacology, Clinical Pharmacology and Toxicology) | Radunović, Nebojša (Clinical Center of Serbia, Belgrade (Serbia). Institute of Gynecology and Obstetrics) | Rajković, Jovana (Faculty of Medicine, Belgrade (Serbia). Institute of Pharmacology, Clinical Pharmacology and Toxicology) | Đokić, Vladimir (Faculty of Medicine, Belgrade (Serbia). Institute of Pharmacology, Clinical Pharmacology and Toxicology) | Petrović, Aleksandar (Faculty of Agriculture, Belgrade-Zemun (Serbia). Institute of Food Technolgy and Biochemistry) | Ivković, Branka (Faculty of Pharmacy, Belgrade (Serbia). Department of Pharmaceutical Chemistry) | Ćupić, Vitomir (Faculty of Veterinary Medicine, Belgrade (Serbia). Institute of Pharmacologyand Toxicology) | Kanjuh, Vladimir (Serbian Academy of Sciences and Arts, Belgrade (Serbia)) | Heinlev, Helmut (University of Tuebingen (Germany). Institute of Physiology) | Gojković-Bukarica, Ljiljana (Faculty of Medicine, Belgrade (Serbia). Department of Pharmacology and Toxicology and Clinical Pharmacology)
Resveratrol is a phytoalexin produced in a number of plant spe- cies including grapes. The benefit of resveratrol to health is widely re- ported. Resveratrol has been found to promote relaxation of non-pre- gnant and pregnant uterus, but its mechanism of action is unclear. The aims of our study were to investigate the involvement of inwardly re- ctifying potassium channels (Kir) in inhibitory effects of resveratrol on three models of contractions of non-pregnant rat uterus: the sponta- neous rhythmic contractions (SRC), oxytocin-elicited phasic contracti- ons and tonic oxytocin-elicited contractions.Uterine strips were obta- ined from virgin female Wistar rats in oestrus. Strips were mounted into organ bath for recording isometric tension in Krebs-Ringer so- lution. Experiments followed a multiple curve design. In order to test the involvement of Kirchannels in a mechanism of action of resvera- trol(1-100 µM),BaCl2 (1 mM),a antagonist of inwardly rectifying pota- ssium channels was used. Resveratrol induced a concentration-de- pendent relaxation of all models of contractions. BaCl2 antagonized the response to resveratrolon SRC and oxytocin-elicited phasic con- tractions. Relaxation achieved by resveratrolon tonic oxytocin-elicited concentrations was insensitive to BaCl2 .The antagonism of resveratrol effects by inwardly rectifying potassium channels antagonist suggests that Kir channels are involved in resveratrol action on phasic contra- ctions of rat uterus. Inhibitory effect of resveratrol on tonic contractions did not include Kir channels.
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