Preliminary assessment of correlation between T-lymphocyte responses and control of porcine reproductive and respiratory syndrome virus (PRRSV) in piglets born after in-utero infection of a type 2 PRRSV
2018
Cha, S.H., Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea | Carey Bandaranayaka-Mudiyanselage, VMRD Inc., Pullman, WA, USA | Chandima B. Bandaranayaka-Mudiyanselage, VMRD Inc., Pullman, WA, USA | Dharani Ajiththos, Washington State University, Pullman, WA, USA | Yoon, K.J., Iowa State University, Ames, USA | Kathleen A. Gibson, Iowa State University, Ames, USA | Yu, J.E., Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea | Cho, I.S., Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea | Stephen S. Lee, University of Idaho, Moscow, USA | Chungwon J. Chung, VMRD Inc., Pullman, WA, USA
A preliminary study into the protective mechanisms of adaptive immunity against porcine reproductive and respiratory syndrome virus (PRRSV) in piglets (n = 9) born to a gilt challenged intranasally with a type-2 PRRSV. Immune parameters (neutralizing antibodies, CD3+ CD4+ , CD3+ CD8+ , CD3+ CD4+ CD8+ T-lymphocytes, and PRRSVspecific interferon (IFN)-γ secreting T-lymphocytes) were compared with infection parameters (macro- and microscopic lung lesion, and PRRSV-infected porcine alveolar macrophages (CD172α+ PRRSV-N+ PAM) as well as with plasma and lymphoid tissue viral loads. Percentages of three T-lymphocyte phenotypes in 14-days post-birth (dpb) peripheral blood mononuclear cell (PBMC) had significant negative correlations with percentages of CD172α+ PRRSV-N+ PAM (p 0.05) as well as with macroscopic lung lesion (p 0.01). Plasma and tissue viral loads had significant (p 0.05) negative correlations with CD3+ CD4+ CD8+ T-lymphocyte percentage in PBMC. Frequencies of CD3+ CD8+ and CD3+ CD4+ T-lymphocytes in 14-dpb PBMC had significant negative correlations with of lymph node (p
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