Anti-inflammatory Effects of Korean Propolis Extracts on Raw264.7 Macrophage Cells
2018
Kim, S.K., National Institute of Agricultural Science, Rural Development Administration, Wanju, Republic of Korea | Woo, S.O., National Institute of Agricultural Science, Rural Development Administration, Wanju, Republic of Korea | Han, S., National Institute of Agricultural Science, Rural Development Administration, Wanju, Republic of Korea | Kim, S.G., National Institute of Agricultural Science, Rural Development Administration, Wanju, Republic of Korea | Bang, K.W., National Institute of Agricultural Science, Rural Development Administration, Wanju, Republic of Korea | Jang, H.R., National Institute of Agricultural Science, Rural Development Administration, Wanju, Republic of Korea | Moon, H.J., National Institute of Agricultural Science, Rural Development Administration, Wanju, Republic of Korea | Kim, H.J., National Institute of Agricultural Science, Rural Development Administration, Wanju, Republic of Korea
We investigated the anti-inflammatory effects of propolis collected from Korea on Raw264.7 macrophage cells. Propolis extract was prepared by ethanol extraction method, the yield of the extract was about 39%. The cell cytotoxicity evaluation of propolis was determined by EZ-cytox cell viability assay. When the cells were treated with propolis concentration of 0, 5, 10, 25, 50, 100, 200 and 1,000 ㎍/ml for cytotoxicity evaluation, the optimal concentration without cell death was 25㎍/ml. We evaluated the anti-inflammatory effect of propolis by nitric oxide (NO) production. To investigate the effect of propolis on the production of NO, a typical inflammatory reaction factor, propolis and lipopolysaccharide (LPS) were treated before, after, and simultaneously, as a result, increased NO production by LPS was shown to be reduced by propolis. The results also showed that the expression levels of inducible nitric oxide synthase (iNOS) and interleukin-1 (IL-1beta) expressed by Western blotting were also consistent. It was confirmed that the expression level of iNOS, IL-1beta increased by LPS was inhibited by propolis and was regulated at the intracellular level. This results show that propolis is controlled at the molecular level in the relief and prevention of the inflammatory reaction, this suggests that propolis is an excellent functional substance in the treatment and prevention of inflammation, also it may be related to the immunomodulation of LPSstimulated Raw264.7 cells.
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