Antioxidant Activities and Cytoprotective Effects of Lonicera japonica Thunb. Extract and Fraction against Oxidative Stress
2018
Lee, Y.S., Seoul National University of Science and Technology, Seoul, Republic of Korea | Yun, M.E., Seoul National University of Science and Technology, Seoul, Republic of Korea | Lee, Y.J., Seoul National University of Science and Technology, Seoul, Republic of Korea | Park, Y.M., Seoul National University of Science and Technology, Seoul, Republic of Korea | Lee, S.L., Seoul National University of Science and Technology, Seoul, Republic of Korea | Park, S.N., Seoul National University of Science and Technology, Seoul, Republic of Korea
In this study, the antioxidant activities and cytoprotective effects against oxidative stress of Lonicera japonica Thunb. 50% ethanol extract and ethyl acetate fraction were investigated. Using the 1,1-diphenyl2-picrylhydrazyl assay, the free radical scavenging activity (FSC50) of L. japonica Thunb. 50% ethanol extract and ethyl acetate fraction was determined as 152.00 and 77.25 μg/ml, respectively. To measure the reactive oxygen species (ROS) scavenging activity, the total antioxidant capacity (OSC50) was determined by using a luminol-dependent chemiluminescence assay. The antioxidant activity of the ethyl acetate fraction (0.33 μg/ml) was approximately four times stronger than that of the 50% ethanol extract (1.12 μg/ml). The protective effect against 1 O2-induced cellular damage of human erythrocytes (τ50) was 46.0 min at 10 μg/ml of the 50% ethanol extract and 52.3 min at 1 μg/ml of the ethyl acetate fraction. We also investigated the cytoprotective effects against oxidative stress induced by H2O2 and the intracellular ROS scavenging activity in response to UVB irradiation and found that the extract and fraction protected human skin cells from damage and reduced ROS. These results confirmed that L. japonica Thunb. was a valuable plant-derived natural antioxidant with potential for development as an antioxidative functional ingredient.
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