Comparison of the stability between branched-chain amino acid (BCAA)-coated liposome and double emulsion
2018
Lee, Y.J., Konkuk University, Seoul, Republic of Korea | Lee, S.Y., Konkuk University, Seoul, Republic of Korea | Shin, H.R., Konkuk University, Seoul, Republic of Korea | Kang, G.H., Konkuk University, Seoul, Republic of Korea | Wi, G.H., Konkuk University, Seoul, Republic of Korea | Ko, E.Y., Konkuk University, Seoul, Republic of Korea | Cho, Y.J., Konkuk University, Seoul, Republic of Korea | Choi, M.J., Konkuk University, Seoul, Republic of Korea
This study was conducted to compare the stability between branched-chain amino acid (BCAA)-encapsulated liposome and double emulsion (DE). Liposome was produced by high-speed homogenization and ultrasonication whereas DE was prepared by homogenizing with surfactants. All samples were fixed at pH 4 and 7 and stored at 4, 25, and 40 ℃ for 5 days. Encapsulation efficiency and cumulative release rate were measured under 4o C and at 25 ℃. The results showed that the size of BCAA-coated liposome was greater at pH 7 than at pH 4. The zeta-potential value of BCAA-coated liposome was greater at pH 4 than at pH 7. It was supposed that the negatively charged liposomes attracted the positively charged BCAAs at pH 4 resulting in the formation of the vesicle with smaller size. Particle size of DE was smaller than 100 μm. Encapsulation efficiencies of BCAA in DE or liposome were over 97%, approximately, and the cumulative release rates of them were below 30% for 5 days.
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