The complete chloroplast genome sequence of Fritillaria thunbergii Miq., an important medicinal plant, and identifcation of DNA markers to authenticate Fritillariae Bulbus
2018
Moon, B.C., Korea Institute of Oriental Medicine, Daejeon, Republic of Korea | Park, I.K., Korea Institute of Oriental Medicine, Daejeon, Republic of Korea | Kim, W.J., Korea Institute of Oriental Medicine, Daejeon, Republic of Korea | Yang, S., Korea Institute of Oriental Medicine, Daejeon, Republic of Korea | Kang, Y.M., Korea Institute of Oriental Medicine, Daejeon, Republic of Korea
The genus Fritillaria is an important herbaceous medicinal plant. The dried bulbs of many Fritillaria species are used as Fritillariae Bulbus in traditional oriental medicines. However, these herbal medicines frequently contain mixtures of diferent types of Fritillariae Bulbus and other Fritillaria species with little or no medicinal value. The accurate identifcation of diferent types of Fritillariae Bulbus is very difcult due to the morphological similarities between the plants and dried bulbs; therefore, it is necessary to accurately identify these herbal medicines and other Fritillaria species based on their genetic characteristics. To fnd molecular markers for Fritillaria species and elucidate the genomic characteristics of F. thunbergii, we sequenced the complete chloroplast (cp) genome of F. thunbergii and performed a comparative analysis with related plant taxa. The complete cp genome of F. thunbergii is 152,155 bp with a quadripartite structure, which is similar to other plants, and was highly similar to previously reported Fritillaria cp genomes in regard to gene content, order, orientation, and GC contents. Comparative analysis of the cp genomes identifed two genes, matK and rps16, that contained sufcient markers to distinguish between two herbal medicines (Fritillariae Thunbergii and Fritillariae Hupehensis Bulbus) and fve Fritillaria species. These results provide useful information for identifcation of Fritillaria species and quality control of Fritillariae Bulbus.
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