Efects of pretreatments on seed dormancy and germination in endemic Uludag fax (Linum olympicum Boiss.) (Linaceae)
2018
Serap Klrmlzl, Bursa Uludag University, Gemlik, Turkey | Gurcan Guleryuz, Bursa Uludag University, Gemlik, Turkey | Hulya Arslan, Bursa Uludag University, Gemlik, Turkey
Linum olympicum Boiss. (Linaceae) is an endemic species to Turkey, and has both medicinal and ornamental properties. In this study, we investigated the efects of GA3 and cold stratifcation (1 or 4 months) on the germination and mean germination time in relation to breaking dormancy in this species under darkness (20 ℃) and light (20/10 ℃, 12/12 h) conditions. Seeds were collected from specimens that spread on limestone or granite screes, ledges of rocky or turfy slopes, and hilltops of the alpine belt from Uludag Mt. (Bursa, Turkey, 2200-2300 m). We show that a combination of hormone and cold stratifcation treatments promoted seed germination under darkness and light conditions. Non-stratifed seeds germinated to 73% following 1000 mg L−¹ GA3 treatment and also it was 83% after 1 month cold stratifcation. After 4 months of cold stratifcation treatment, 10% and 53.3% of seeds successfully germinated after incubation in light and dark conditions, respectively. Hormone and cold stratifcation treatments also reduced mean germination time. Our results revealed that seeds of L. olympicum have physiological dormancy and require prolonged time for cold stratifcation and darkness for germination, rather than responding to the photoperiod. Germination requirements of L. olympicum have not been studied previously, and the data obtained could be used for the ex situ protection of this alpine species.
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