Identification of variety-specific alleles and loci in Philippine citrus collection using simple sequence repeat (SSR) markers
2017
Tanogbanua, K.A, | Espino, R.R.C
DNA profiles of 44 citrus varieties from nine species were constructed using 44 polymorphic simple sequence repeat (SSR) markers to characterize and efficiently identify citrus species and varieties in the Philippines. The SSR-derived similarity coefficients of the collection ranged from 0.43 to 0.77, which was deemed sufficient to delineate the varieties. SSR polymorphism information content (PIC) values were moderate to high, ranging from 0.31 to 0.98, thus proving that SSR is a robust marker for variety identification. Variety-specific markers were identified to systematize and hasten variety identification and authentication. This is the first study using molecular markers to develop an identification key for citrus in the Philippines for faster breeding for commercial purposes.
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