Effect of methyl jasmonate on the expression of phenylalanine ammonia lyase and eugenol-o-methyl transferase genes in basil
2017
Milan, E.B. | Mandoulakani, B.A. | Kheradmand, F.
Basil (Ocimum basilicum L.) is one of the important medicinal plants belonging to the Lamiaceae family used as fresh herb. Methyl jasmonate (MeJa) is a hormone signal and endogenous growth regulator involved in the regulation of defense responses, which induces a broad range of physiological pathways in many plant species. In this study, a completely randomized design (CRD) with three replications was conducted in a greenhouse to evaluate the effect of MeJa on the expression of eugenol-o-methyl transferase (EOMT) and phenylalanine ammonia lyase (PAL) genes (key genes involved in the biosynthesis of phenylpropanoids) in basil. Two concentrations of Meja (0 and 0.5 mM) were sprayed on healthy plants at pre-flowering stage. Plant leaves were sampled at 0,24,48, and 72 h after MeJa application, and the expression of the EOMT and PAL genes was studied using real time polymerase chain reaction (PCR). Results showed that MeJa with 0.5 mM concentration significantly increased the expression of both genes. The expression of both genes reached its maximum amount 48 h after MeJa application, but the expression of the PAL gene significantly declined after that. In conclusion, it was demonstrated that the external application of MeJa could significantly induce the expression of EOMT and PAL genes in basil.
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