In vitro propagation of plum rootstocks
2018
Vujović, Tatjana | Marjanović, Tatjana | Ružić, Đurđina | Glišić, Ivana
To determine the effect of successive subculturing on multiplication capacity of shoots, three rootstocks for plum, i.e. Cherry plum (Prunus cerasifera Ehrh.), ‘Docera 6’ (P. domestica × P. cerasifera) and ‘Dospina 235’ (P. domestica × P. spinosa) were repeatedly subcultured for five subcultures on Murashige & Skoog (MS) medium of unchanged plant growth regulator composition. Monitoring of the shoot formation capacity revealed constant increase in multiplication index and length of axial and lateral shoots in Cherry plum during each consecutive multiplication cycle. As for ‘Docera’ and ‘Dospina’, significan increase in multiplication index and length of axial shoots was observed in the third subculture. After that their values gradually decreased to the fifth subculture, but remained considerabely higher in comparison with the values in the first two subcultures. This study has confirmed that response of in vitro cultures to constant subculturing is genotype dependant. Considering that the decline in multiplication index was already observed after third subculture, it is neccesary to determine when cytokinin type and concentration should be reduced, or the hormon-free medium should be deployed to delay the decrease. MS medium with mineral salts reduced to ½-strength and organic complex unchanged was used in rooting stage. The highest capacity for in vitro rooting was observed in Cherry plum (100%), followed by ‘Docera’ (91.7%), while the lowest was in ‘Dospina’ (51.9%). Therefore, an additional optimization of this stage by testing other types and concentration of auxin should be done in order to achieve higher rooting percentage in ‘Dospina’.
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