Phylogenetic analysis of salmonella infantis strains from poultry by mlst

20 strains of Salmonella Infantis isolated in the scope of the 113R036 TÜBİTAK project in Ankara University Veterinary Faculty Microbiology Department between 2014-2017 were typed with MLST. Six different serotypes, S. Enteritidis, S. Agona, S. Typhimurium, S. Hadar, S. Mbandaka and S. Coeln, were used to show the evolutionary relationship between different sequence types and serotypes on phylogenetic tree. For each strain 7 housekeeping gene (aroC, dnaN, hemD, hisD, purE, sucA and thrA) were amplified using the primers indicated in the Pubmlst database. DNA sequence analysis was performed with sequence analysis primers from the database and gene sequences were determined. For each strain, sequence type was determined on Pubmlst database with obtained sequences. 7 genes sequences (501 bp for aroC, 501 bp for dnaN, 432 bp for hemD, 501 bp for hisD, 399 bp for purE, 501 bp for sucA and 501 bp for thrA) were combined to obtained 3336bp seqeunce based on a strain. A phylogenetic tree was constructed based on 26 DNA ssequences with T92 displacement model and NJ phylogenetic tree construction method.All S. Infantis strains were found sequence type 32, external strain strains that S. Enteritidis, S. Agona, S. Typhimurium, S. Hadar, S. Mbandaka and S. Coeln respectively were found sequence type 11, 13, 19, 33, 413 and 2015. For all serotypes, the most common and dominant sequence types by the country and around the world were also identified in this study. Considering similar studies in our country and around the world in the past years, it has been understood that there are dominant and globally accepted sequence types for the serotypes studied and that these dominant sequence types have not changed in the course of local studies. Taking into account the vaccines consisting of sequence type-based components which are being used for various bacterial strains, we concluded that the ST32 sequence type s should be taken in a vaccination for S. Infantias in our country. In the tree showing the evolutionary distance between 7 different sequence types, the closest sequence types were 32 and 19 (S. Infantis and S. Typhimurium). In future studies, it is concluded that the probability of finding other sequence types of S. Infantis serotype increases when the number of samples is increased, and that these can be placed in different taxa in the phylogenetic tree.