Obtaining and characterisation of cell wall surface biopolymers preparations of non-pathogenic corynebacteria
2014
I. Furtat
In this paper we obtain and characterize the cell wall surface biopolymers preparations of nonpathogenic corynebacteria. The method of extraction from whole cells with 1 % solution of sodium dodecyl sulfate was shown to be effective to obtain Corynebacterial cell wall surface biopolymers preparations. It has been established, that following the surface biopolymers extraction,Сorynebacterial cells do not lyze, remain viable, do not change Gram staining, and contain the main structural component of the cell wall like complex of peptidoglycan-arabinogalactan-mycolic acids. It was also shown that acidic amino acids weredominant in the cell wall surface biopolymers preparations, neutral and basic amino acids were also present, but in smaller numbers. Trace amounts of sulfur-containing amino acids (cysteine and methionine) were present in SDS-extracts, which is typical for the vast majority of surface proteins of bacteria. Electrophoretic spectra of surface biopolymers were investigated,on the basis of which, preparations obtained from 24 hour cultures were recommended for comparative studies of surface biopolymers of different species of actinobacteria.
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