Determination of organic acids in honey samples from Latvian market by high-performance liquid chromatography
2019
Keke, A., Latvia Univ. of Life Sciences and Technologies, Jelgava (Latvia) | Cinkmanis, I., Latvia Univ. of Life Sciences and Technologies, Jelgava (Latvia)
Determination of organic acids in honey samples from Latvian market by high-performance liquid chromatography Honey is a naturally sweet product, which is produced by honeybees (Apis mellifera). Honey is a natural source of antioxidants and has been known to mankind since ancient times. Honey contains approximately 200 different compounds. Organic acids can be used as an indicator to detect the freshness, authenticity and acidity of honey. The aim of this research was to determine and quantify organic acids such as oxalic, L-tartaric, D-quinic, L-malic, L-ascorbic, citric, fumaric and succinic in honey samples from Latvian market u sing high-performance liquid chromatography. The chromatographic separation of organic acids was carried out with PerkinElmer C18 (4.6 mm Í 250 mm I.D, particle size 5 mm) analytical column at the temperature of 35 °C in wavelength at 210 nm. The obtained results showed that the analysed honey samples contain L-tartaric, D-quinic, L-malic, L-ascorbic, citric, fumaric and succinic acids. The concentration of these acids was found to be variable. Oxalic acid was not detected in the analysed honey samples. L-tartaric acid was the main acid in all analysed honey samples.
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