Molecular screening of abaca (Musa textilis L. Nee) accessions using microsatellite markers associated with resistance to bunchy top virus
2016
Boguero, A.P.B. | Parducho, M.A.L. | Mendoza, M.R.D. | Abustan, M.A.M. | Lalusin, A.G.
Fifty seven abaca accessions of the Institute of Plant Breeding [University of the Philippines] were assessed using 6 SSR markers associated with resistance to determine their genetic diversity, identity resistant and susceptible accessions for bunchy top virus, and establish a basis for construction of a genetic base pool in breeding for resistance to bunchy top virus. Eighteen accessions were identified disease-free, 24 accessions showed mild resistance, 9 accessions were severely affected by bunchy top virus, and 6 accessions have no rating. All the accessions obtained from Palawan were observed healthy. The 57 accessions are highly diverse with Shannon-Weaver index of 0.92 and polymorphism information content value was 0.61. Accessions were grouped into 10 distinct groups with 41% Jaccard's similarity coefficient. Abuab Labo (resistant) and Maguindanao Pacol (resistant) have 56% and 45% similarity to Pacol, respectively, a source of resistance to bunchy top. Principal component analysis displayed a widely diverse set of population where each resistant accession is distributed throughout the four panels. The diversity of the accessions revealed that there would be other possible sources of resistance genes aside from Pacol which could be used for further improvement of abaca resistance.
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