Diversity analysis of Philippine citrus collection using simple sequence repeat markers
2018
Tonogbanua, K.A. | Espino, R.R.C. | Espino, M.R.M.
Genetic diversity analysis of 44 citrus varieties in the Philippines using 44 SSR markers was done to assess the degree or relatedness and elucidate genetic relationship between and within species. Markers used were characterized and optimized resulting to 248 clear amplicons and at most 14 diverse banding patterns. Polymorphism information content (PIC) values were 0.31-0.98, proving the effectivity of SSR in variability detection. All the varieties were distinct from one another with at most 77% similarity. Two types of clusters were observed with bootstrap values 89-100, clusters with C. grandis and clusters with mixed members (mostly C. sinensis and C. reticulata). Monoembryonic species have a narrow genetic base as compared to the polyembryonic species and thus clustered exclusively.
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