Analysis of Cellular Responses to Native and Recombinant Proteins of Cowdria ruminantiumaa
2006
albert bensaid | philippe totté | antony barbet | duncan mwangi | declan mckeever | frans jongejan | suman m. mahan
PHILIPPE TOTTÉ et al., 'Analysis of Cellular Responses to Native and Recombinant Proteins of Cowdria ruminantiumaa', Annals of the New York Academy of Sciences, vol. 849(1), pp.155-160, Wiley, 2006
显示更多 [+] 显示较少 [-]The response of peripheral blood mononuclear cells (PBMC) collected from cattle immunized with killed C. ruminantium to MAP1, MAP1-B, MAP2 and to a lysate of Cowdria was characterized in vitro. CD4+ T lymphocytes responded strongly to Cowdria lysates while B cells and gamma delta T cells proliferated in response to recombinant proteins. Short-term cell lines derived from PBMC using Cowdria lysates were >90% CD4+ and did not respond to any of the recombinant proteins. Restimulation of PBMC with MAP1, MAP1-B, and MAP2 proved difficult, and cultures usually stopped proliferating after 2 to 3 weeks. Only 1 cell line could be generated using MAP1 and it was composed of 85% gamma delta T cells. This study indicates that in animals immunized with killed Cowdria, proteins other than the serologically immunodominant MAP1 and MAP2 are strongly immunogenic for T lymphocytes
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