Selection and evaluation of potential reference genes for gene expression analysis in Avena fatua
2019
Liu, Junjie | Li, Peng | Lu, Liuyang et al.
Eight commonly used candidate reference genes, 18S ribosomal RNA (rRNA) (18S), 28S rRNA (28S), actin (ACT), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), elongation factor 1 alpha (EF1?), ribosomal protein L7 (RPL7), Alpha-tubulin (?-TUB), and TATA box binding protein-associated factor (TBP), were evaluated under various experimental conditions to assess their suitability in different developmental stages, tissues and herbicide treatments in Avena fatua. The results indicated the most suitable reference genes for the different experimental conditions. For developmental stages, 28S and EF1? were the optimal reference genes, both EF1? and 28S were suitable for experiments of different tissues, whereas for herbicide treatments, GAPDH and ACT were suitable for normalizations of expression data. In addition, GAPDH and EF1? were the suitable reference genes.
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