[Retracted] Effect of cadmium on resumption of respiration in cotyledons of germinating pea seeds
2010
Smiri, Moez | Chaoui, Abdelilah | Rouhier, Nicolas | Jacquot, Jean-Pierre | El Ferjani, Ezzedine
Pea seeds (Pisum sativum L.) were germinated by soaking in H2O OR 5mM CdCl2 during a 5-day period. Enzyme activities involved in respiratory metabolism were studied in cotyledons. Mitochondrial cytochrome c oxidase and NADH- and succinate-cytochrome c reductase activities wer inhibited by cadmium treatment. The effects of Cd were performed in vivo and in vitro allowing to distinguish between the direct inhibition of the enzyme activities and the influence of the same enzymes into the cell environment. However, Cd exposure stimulated an enzyme activity of fermentation and inhibited the capacity of the enzyme inactivator (alcool dehydogenase inactivator). Moreover, the enzyme activities of NAD(P)H-recycling dehydogenases via secondary pentose phosphate pahtway, glucose-6-phosphate- and 6-phosphogluconate-dehydrogenases, were enhanced in Cd-stressed seeds. The findings will help clarify the overall mechanisms that underlie cadmium-mediated toxicity in germinating seeds.
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