Silica nanoparticles-assisted electrochemical biosensor for the rapid, sensitive and specific detection of Escherichia coli
2019
Mathelié-Guinlet, Marion | Cohen-Bouhacina, Touria | Gammoudi, Ibtissem | Martin, Axel | Beven, Laure | Delville, Marie-Hélène | Grauby-Heywang, Christine
To face the emerging issues raised by bacterial infections in biomedical, food and environmental contexts, the combination of nanotechnologies and biosensors characteristics is increasingly seen as a promising tool to develop rapid, highly sensitive and specific devices for reliable bacterial detection and quantification. We present here the design of an electrochemical immuno-biosensor, integrating non cytotoxic silica nanoparticles (NPs), in a two-step spin coating process. Unlike biosensors that integrate a unique antibody-based layer without NPs, this new tool allows, through cyclic voltammetry measurements, the continuous and non-saturated detection of E. coli bacteria in only 5 min. and can reach up to 103 CFU/mL in 30 min. It offers great promises for industrial purposes as electrode functionalization is rather simple and its architecture can be tuned on demand to target a variety of micro-organisms, and the highlighted indicators (variations of current intensity, intensity ratios and/or difference of potentials) can easily be implemented in a screen displayed packaging.
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