Analysis of the Genetic Effects to Frogs (Fejervarya limnocharis) After Acute Lead Exposure In Vivo

This study aimed to investigate the bioaccumulation and elimination of lead (Pb) in Fejervarya limnocharis frogs as well as to determine the genotoxic effects of direct Pb exposure at different concentrations and lengths of time. Four varying concentrations (0, 5, 10 and 20 mg.L-1) of lead acetate (Pb(CH3COO)2) solutions were injected intraperitoneally into F. limnocharis. The concentration of Pb in the water samples used to house the frogs and the concentration of lead in frog muscle tissues were analysed at 24, 48 and 72 hours after injection by inductively coupled plasma optical emission spectrometry. Pb was detected at a level that exceeded the standard (0.03 mg.kg-1) in all samples of frogs injected with Pb. The water samples indicated that the Pb concentrations were significantly different from the control (p < 0.05), except for the 5 and 10 mg.L-1 concentrations after 24 hours groups and 5 mg.L-1 concentration after 48 hours group. Only the concentration of the water in the 20 mg.L-1 for 72 hours group exceeded the standard (0.05 mg.L-1). Genetic differentiation was studied by inter simple sequence repeats (ISSR) with dendrogram construction and analysis of genetic similarity (S) for each duration of exposure. A total of 1158, 1205 and 1277 bands were generated by ISSR for the 24, 48 and 72 hours groups, respectively. In each dendrogram, individual injections with the same Pb concentration clustered together, and it appeared that higher concentrations resulted in greater genotoxicity. Genotoxicity was concentration- and time-dependent, with a correlation between the concentration and S-value for the 72 hours group (R2 = 0.77, p < 0.05). In addition, this study could provide a basic application to develop F. limnocharis as a biomarker for Pb contamination by measuring genotoxic consequences.