Mendelian inheritance of microsatellite markers in Southeast Asian river catfish, Mystus nemurus

Hoh, B. P.; Siti Shapor Siraj; Tan, S. G.; Khatijah Yusoff

Mendelian inheritance of microsatellite markers in Southeast Asian river catfish, Mystus nemurus

2005

Hoh, B. P. | Siti Shapor Siraj | Tan, S. G. | Khatijah Yusoff

Eight previously designed polymorphic microsatellite loci, namely, Mnc434a, Mnc65b, Mnc441, MnBp5-1-02b,MnBp5-1-30b, MnBp5-2-02b, MnBp8-4-43a, and MnBp8-4-43b, were used to examine their modes of segregationin Mystus nemurus family study. Randomly selected broodstocks from Terengganu population were used togenerate F1 population. DNA was isolated from tissue samples of the parents, whilst the whole one-month-old F1progeny was used to isolate the DNA. Chi-square analysis was performed to test the significance of the resultsobtained. It was shown that all the eight microsatellite loci examined segregated close to Mendelian inheritanceratios, which would be useful in generating a genetic linkage map for this species.

显示更多 [+]