Isolation and characterization of Lactobacillus strains as potential probiotics for chickens
2014
Shokryazdan, P. | Kalavathy, R. | Sieo, C. C. | Alitheen, N. B. | Liang, J. B. | Jahromi, M. F. | Ho, Y. W.
The ban on the use of antibiotics as growth promoters for poultry production in many countries has led to increasing interest to use probiotics as an alternative. In the present study, someLactobacillus strains were isolated from chicken intestines, identified and assessed (in vitro) for their ability to survive and colonize the gastrointestinal tract (GIT), with a view to select suitable strains as potential probiotic candidates for chickens. Outof 42 isolated strains, three isolates, identified as Lactobacillusstrains based on initial identification and tolerant to acid and bile based on preliminary screening using turbidity (optical density) as a measurement of growth, were selected for detailed identification and further in vitro assays. The three isolates were identified to species level using carbohydrate fermentation profile analysis and 16S rRNA gene sequencing. Results showed that allthree strains belonged to Lactobacillus salivarius . The threeL. salivarius strains were then assessed for their ability to tolerate the stress conditions in the GIT and capacity to adhereto the intestinal epithelial cells using in vitroassays of acid, bile and pancreatic enzyme tolerance measured by viable colony counts, and adhesion assay using Caco-2 cell line. Theresults showed that all three L. salivarius strains exhibited good tolerance to acid, bile and pancreatic enzymes and a strong abilityto adhere to intestinal epithelial cells. Thus, they would be able to survive the stress conditions of GIT, as well as to attach andcolonize the GIT, and could be considered as good potential candidates for probiotics of chickens.
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