Optimization of phenolic compound recovery and antioxidant activity from carob pulp using response surface methodology
2017
Saci, F. | Louaileche, H. | Bachir bey, M. | Meziant, L.
The optimum extraction conditions for recovery of total phenolics and antioxidant activity of carob pulp were analysed using response surface methodology. The effects of acetone concentration (60-80%), extraction time (60-120 min), extraction temperature (50-90°C) andsample/solvent ratio (10-40 mg/10mL) were investigated. Second order polynomial models produced a satisfactory fitting of the experimental data with regard to total phenolic content (R2 = 0.9949, P<0.0001) and antioxidant activity (R2 = 0.9947, P<0.0001). Response surfaceanalysis showed that the optimal extraction parameters that maximized antioxidant extraction were acetone concentration of 70.85%, extraction time of 101.50 min, temperature of 88.35°C and sample/solvent ratio of 16.65mg/10mL. The optimum values for total phenolic and antioxidant activity were 2557.96 mg GAE/100g and 2393.75mgGAE/100g, respectively. The experimental values agreed well with those predicted, indicating the suitability of the modeland the success of response surface methodology in optimizing the extraction conditions.
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