Bioproteins production from palm oil agro-industrial wastes by Aspergillus terreus UniMAP AA-1
2016
Khadijah Hanim Abdul Rahman | Siti Jamilah Hanim Mohd Yusof | Zarina Zakaria
Presently, the animal feed industry is suffering from inadequate and expensive conventional protein ingredients due to the increasing demand for food and feed products. This has ledto the search for unconventional protein sources to fulfil market needs. In this study, the potential of selected palm oil wastes, namely palm pressed fibre (PPF) and palm oil decantercake for bioprotein production, was investigated. Fermentation process was carried outaerobically in conical flasks with the working mass of 20 g each at 32°C for seven days.The performance of these palm oil wastes as substrates in solid state bioconversion ofAspergillus terreus UniMAP AA-1 strain were evaluated. A substrate with higher proteinyield was chosen for the subsequent parameter screening using 2-level factorial design.Results showed that the protein content in PPF and palm oil decanter cake was increased upto 401 mg/L and 493 mg/L, respectively post-fermentation. Among the parameters studied,substrate concentration and inoculum size were found to significant affect bioproteinproduction. The highest protein content of 1683 mg/L was successfully produced frompalm oil decanter cake at temperature of 35°C with 50% substrate concentration and 15%of inoculum size, suggesting its potential as an alternative protein source. Thus, this studyprovides preliminary data for future process optimisation of bioprotein production usingthe statistical approach.
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