Biomass production by Yarrowia lipolytica as a source of lipids: bench scale cultivation on raw glycerol-based medium
2015
Dalcanton, F. | Michelon, M. | Furlong, E. B. | Burkert, J. F. M. | Machado Junior, F. R. S. | Burkert, C. A. V.
This paper analyzes the aeration and agitation effects in growth parameters and lipid content for Yarrowia lipolytica NRRL YB-423 growing in a raw glycerol-based medium. A central composite design (22 assays plus three central points) was made, with combinations of agitation (100 to 300 rpm) and aeration (0.5 to 1.5 vvm). The resulting KLa values ranged from 2.25 to 16.83 h-1. The most favorable condition was 200 rpm and 1 vvm (KLa of 8.29), producing 19.14 g L-1 biomass with a lipid content of 7.87%. In relation to fatty acid profile, polyunsaturated fatty acids prevailed (62.55%), achieving 49.16% linoleic acid (C18:2). Therefore, this yeast biomass has the potential to constitute a sustainable and renewable alternative as a source of essential fatty acids in food formulations for animals and as a raw material for the development of glycerol-based biorefineries for fuels and chemicals.
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