Effect of repeated laparoscopic ovum pick-up on yield and quality of oocytes
2015
Nor Farizah A. H. | Rahman M. M. | Wan Khadijah W. E. | Abdullah R. B.
Laparoscopic ovum pick-up (LOPU) is a less invasive surgical technique for oocyte recovery from antral follicles seen as ‘pimple-like’ protrusion on the ovarian surface. LOPU can be repeated several times without ovarian damage or reducing the doe’s fertility. This study investigated the effect of LOPU cycles on yield and quality of oocytes in goats. Three repeated cycles (oocyte retrieval 1 (OR1), oocyte retrieval 2 (OR2) and oocyte retrieval 3 (OR3)) of LOPU procedure were performed on 16 donor Boer crossbred does. The oocytes obtained were classified based on cumulus oocytes complexes (COC) layer criterion into 5 grades: grades A, B, C, D and E. OR1 gave a higher number of quality oocytes (P<0.05), followed by OR2 and OR3 of 5.47, 3.94 and 3.09 oocytes per ovary, respectively. Number of oocytes with grades B and D was not significantly different (P>0.05) among the OR cycles. The number of grade A oocytes obtained in OR1 and OR2 was significantly higher (P<0.05) than that in OR3 whereas the number of grade C oocytes obtained in OR1 was significantly higher (P<0.05) than those in OR2 and OR3. Grade E oocytes were significantly higher (P<0.05) in OR2 and OR3 compared to OR1. Percent recovery of grade C oocytes was higher (P > 0.05) than for grades A and B oocytes with mean oocyte recovery rate of 153 versus 106 and 91%, respectively. In conclusion, LOPU is a reliable procedure to provide consistently high quality oocytes of grades A, B and C and its usage can be repeated on the same donor doe for up to 3 times. However, the number of oocytes is reduced following each oocyte retrieval cycle.
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