Polymorphism of Insulin-like Growth Factor 1 gene in Kedah-Kelantan cattle using PCR-RFLP technique

Suriaty, R.; Mastura, Y.; Mohd Hafiz, A.R.; Mohd Hafizal, A.

Polymorphism of Insulin-like Growth Factor 1 gene in Kedah-Kelantan cattle using PCR-RFLP technique

2015

Suriaty, R. | Mastura, Y. | Mohd Hafiz, A.R. | Mohd Hafizal, A.

The Kedah-Kelantan cattle (KK) is an indigenous cattle breed and is mainly kept for meat production in Malaysia. Due to lack of information about polymorphism of growth traits in these cattle, Insulin-like Growth Factor 1 (IGF-1) was chosen to be the candidate gene in this preliminary study. The aim of this study is to investigate the polymorphism of IGF-1 gene in KK and to show that the PCR-RFLP technique can be used as a basis for use as molecular markers in cattle. A total of 46 KK blood samples were collected for DNA extraction performed using a commercial kit. The exon 1 of IGF-1 gene was amplified to produce a 249 bp fragment. The amplified fragments were digested with Eco105I restriction endonuclease and then subjected to electrophoretic separation in Fluorosafe stained 2.5 % agarose gel. The result revealed two alleles, A and B. Threegenotypes were observed: AA, AB and BB. Frequencies were 0.07, 0.13 and 0.80 for AA, AB and BB, respectively. This gives frequencies of 0.13 and 0.87 for A and B alleles. It is concluded that the population is in Hardy-Weinberg disequilibrium (p<0.05). It is possible that this gene has been exposed to selection.

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