Somatic embryogenesis from petiole explant of papaya (Carica papaya L. Cv. Eksotika)
2004
Panjaitan S. B. | Aziz M. A. | Rashid A. A. | Saleh N. M.
An experiment was carried out to induce somatic embryogenesis from petiole explants of papaya cultivar Eksotika using different concentrations and combinations of 2.4-D and BAP. Petiole explants were cultured on modified MS medium with half-strength macro nutrients and supplemented with 500 mgL-¹ glutamine, 0.2% phytagel and 1.0, 2.5, 5.0 and 7.5 mgL-¹ 2,4-D in combination with 0, 0.001, 0.005 and 0.010 mgL-¹ BAP. Treatment containing 7.5 mgL-¹ 2,4-D without BAP produced the highest percentage of callus formation (89%). Highest percentage of somatic embryo formation (20%) occurred in treatment containing 5.0 mgL-¹ 2,4-D. In this treatment the mean number of somatic embryos produced per explant was 12.
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