Antioxidant activity of bee pollen ethanolic extracts from Malaysian stingless bee measured using DPPH-HPLC assay
2016
Nurdianah H. F. | Ahmad Firdaus A. H. | Eshaifol Azam O. | Wan Adnan W. O.
Bee pollen is considered as one of the functional foods due to its complex biochemical properties. Bee pollen which is collected from pollen grains from various botanical sources contains almost a complete nutrition such as carbohydrates, proteins, amino acids, vitamins and minerals. Its beneficial effect on health is thought to be due to the presence of phenolic compounds with its antioxidant activity. Antioxidant activities of ethanolic bee pollen extract (BPE) from three species of Malaysian stingless bee; Trigona thoracica, Trigona itama and Trigona apicalis in this study were measured using DPPH-HPLC method and gallic acid (GA) as a standard reference. The percentage of DPPH inhibition by T. apicalis BPE at 1 mg/mL showed the highest inhibition (39%, GA equivalent to 0.3 mg/mL) compared with T. itama (14.3%, GA equivalent to 0.1 mg/mL) and T. thoracica (6.7%, GA equivalent to 0.05 mg/mL). Our result was the first in reporting antioxidant activity of BPE measured using DPPH-HPLC method from three different species of Malaysian stingless bee.
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