Identification of methicillin-resistant staphylococci isolated from milk and milk products of Southern Assam, India and evaluation of their enterotoxigenicity
2017
Mazumder, P. B. | Mohanta, A. | Bhattacharjee, A.
In this study, methicillin-resistant staphylococci isolated from milk and milk products were evaluated for their enterotoxigenic potential with emphasis on the three classical enterotoxin genes namely, SEA, SEB and SEC. 11(35.48%) out of 31 staphylococcal isolates were found to be methicillin-resistant, being positive for mecA gene. Of these 11 methicillin-resistant isolates, 8 (72.73%) were positive for either SEA or SEC. Sequencing of the 16S rDNA helped identify the methicillin-resistant isolates as S. aureus, S. sciuri, S. epidermidis and S. saprophyticus. Phylogenetic tree constructed with the 16S rDNA sequences revealed a close relationship among the isolates that accounts for dissemination of genes like those for antibiotic resistance and enterotoxins.The presence of methicillin-resistant enterotoxigenic strains of Staphylococcus in popular food products like milk and milk products analysed in this study indicates potential health risk for consumers and emphasizes the necessity of hygienic handling of these food products.
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