Isolation and expression of a heat shock protein gene during ripening and storage of papaya fruit
2000
Razean Haireen Mohd Razali | Zainon Mohd Ali | Hamid Lazan
Postharvest heat treatment, which may induce chilling injury tolerance in papaya (Carica papaya L cv Eksotika), may possibly affect the expression of the different class of heat shock protein (HSPs). In this study, a cDNA library prepared from ripening papaya fruits were screened at reduced stringency with pTOM66, a clone encoding small molecular size, tomato smHSP. A positive cDNA clone of about 1.5 kb in length was isolated and analyzed, and showed homology to an unknown protein of Arabidopsis thaliana. Expression of this hypothetical papaya HSP (carHSP) gene during normal ripening and storage of papaya at chilling temperature (10°C) was investigated by Northern blotting. Results indicate that expression of carHSP decreased through normal ripening at ambient, but the levels of the mRNA transcripts, particularly in those fruits that have been subjected to heat-treatment, increased during storage at low temperature (LT). Transferring the LT-stored fruits to ambient resulted in an initial suppression of carHSP expression. but later, the gene activity seemed to recover.
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