UV-Vis spectroscopy and chemometrics as a tool for identification and discrimination of four Curcuma species
2018
Kautsar, A. | Jannah, R. | Septaningsih, D. A. | Rafi, M. | Heryanto, R.
Curcuma species such as Curcuma longa (turmeric), C. xanthorrhiza (java turmeric), C. aeruginosa (black turmeric) and C. mangga (mango ginger) are widely used in jamu, Indonesian traditional medicines as well as herbal drink and food supplement. The identification and discrimination of these closely-related plants is a crucial task to ensure the quality and to prevent adulteration of their raw materials. Therefore, we developed a feasible and rapid method using UV-Vis spectra in combination with chemometrics for discrimination of the four species. Firstly, we extract all of the samples using sonication method with methanol as the solvent for 40 minutes. The UV-Vis spectra of this four species were acquired in the interval of 200-800 nm and then standard normal variate was used for preprocessing the spectral data. Principal component analysis (PCA) and discriminant analysis (DA) were used for classification of the four species. It turned out that the discrimination of the four species was achieved through the combination of the pre-processed UV-Vis spectra with PCA and DA, in which DA gave clearer classification according to the species because of 95.5% of the sample correctly classified into their groups by leave-one-out-cross-validation.
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