Latex B-serum b-1,3-glucanase (Hev b II) and a component of the microhelix (Hev b IV) are major latex allergens
1995
Ward, M. A. | Samsidar Hamzah | Yeang, H. Y. | Sunderasan E. | Sharifah Hamid | Cardosa, M. J.
Two allergenic (IgE-binding) proteins have been isolated from the B-serum of natural rubber latex by dialysis-induced protein precipitation followed by gel filtration. One of the allergens, Hev b II, that is specific to the + monoclonal antibody USM/RB4, commonly appears as a doublet of molecular weights 34 and 36 kDa under reducing conditions of SDS-polyacrylamide gel electrophoresis. In the absence of 2-mercaptoethanol, a 70 kDa band can also be seen in addition to the + doublet. The allergen has a pi of circa 9.5 and is identified as b-l,3-glucanase. The second allergen, Hev b IV, has a pI at circa 4.5 and appears under reducing conditions as a band of 50-57 kDa. In its unreduced form, a principal band of approximately + 100 kDa is discernible. Immuno-gold labelling with the monoclonal antibody USM/RB3 shows that Hev b IV is a component of the microhelix protein complex of B-serum.
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