The effect of different combinations of cytokinins on shoot proliferation of plant micropropagation in mangosteen (Garcinia mangostana L.)
2003
Rahayu S. | Kadir M. A. | Aziz M. A. | Rashid A. A.
Mangosteen (Garcinia mangostana L.) is an under exploited tropical fruit tree with promising economic value. Propagation of mangosteen is commonly by seeds Mangosteen fruits seasonally and produce seeds at the rate of two seeds per fruit. The development of mangosteen plant is slow and it takes about 10 - 12 years to bear fruit. The seeds are known as recalritrant. The recalritrant behavior causes difficulties in producing planting materials throughout the year. Up to now, there is no suitable method for storing mangosteen seeds. Thus, there exists a problem of not having enough planting materials for large scale planting. The conventional vegetative propagation method has low percentage of success. One of the alternative approaches for mass propagation of mangosteen is through tissue culture. With this technique, large number of seedlings can be produced in a short time. By the method hopefully shoots can be regenerated. Thus, the requirement of planting materials for mangosteen can be fulfilled. Shoot proliferation from seed segments of mangosteen has been conducted. Multiplication was achieved by culturing seed segments on Murashige and Skoog medium enriched with various concentrations of cytokinins, i.e.: BAP, kinetin and 100 mg/l Adenine sulfate in combination or alone, 30 g/l sucrose, 3.9 mg/l Gelrite agar and kept 8 weeks in culture. Shoots were obtained in all those media after 2 weeks in culture. After 8 weeks in culture, there were significantly different among the treatments used on the induction of shoot. The best media for shoot proliferation was 8 mg/l BAP (11.82 shoots per explant) and the highest shoot was obtained from 1 mg/l kinetin (2.27 cm).
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