Radiosensitivity of Microsorum sp: a fern species through in-vitro propagation
2002
Azhar M. | Amran A.H. | Rusli I.
Microsorum sp. is a beautiful olive green fern species that grows from a horizontal rhizome. In creating variability for plants, mutation induction is an alternative way to change plant charatecristics. Radiosensitivity test is an important factor in deciding the approach to be taken for genetic changes of the fern in mutation breeding. Plantlets of Microsorum sp. were irradiated at doses of 0,10,20,40,60,80,100 and 150 Gy using a gamma cell with 60Co source at dose rate of 0.25 Gy s-1. Irradiated plantlets were cultured on semi solid modified hormone free Murashige and Skoog (1/2 strength MS) media and incubated at 24C with a photoperiod of 16 hours (3500 lux). Radiosensitivity and growth performance assessed by survival and multiplication rate 4-5 weeks after the treatment. Increasing gamma ray doses caused reduction in survival rate as well as the number of new leaves produced per plantlet. LD50 for Microsorum sp. was 73Gy, as estimated from the dose rate vs. survival curve. Regenerated plantlets from treated plantlets showed a slow recovery growth, especially at higher doses as compared to control plantlets.
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