Allele mining of allele-specific SNP/indel markers of yield related traits in Philippine rice (Oryza sativa L.) landraces
2019
Badajos, A.T. | Enriquez, J.O.S. | Caguiat, J.O.
Improvement of rice grain yield is made possible by accumulation of beneficial alleles from plant genetic resources through marker assisted selection. Allele mining is an important molecular approach towards identification of allelic diversity that affects plant phenotype and vital for the introgression of useful alleles for efficient utilization of genetic resources in plant variety development. Two hundred forty Philippine modern and traditional rice genotypes were evaluated to mine yield related genes and their useful alleles for rice grain number, grain size, 1000-grain weight, spikelet number, tillering, panicle branching, plant height and heading date with the objected to assess presence and diversity of established yield enhancing genes and analyze its effects. Eight reported genes ( Gn1a, SCM2, Ghd7, SPIKE, GS5, OsSPL14 and TGW6) associated with rice grain yield were used for PCR amplification. It was found that Gn1a, SPIKE, OsSPL14 and Ghd7 can be used in identifying alleles enhancing grain number, spikelet number, plant height and heading date from a divers genomic resources. Gn1a-indel1 (99 bp) of Gn1a and Ghd7-05snp (201 bp) of Ghd7 associated with grain number, plant height and heading date were the most abundant alleles across the evaluated panel and can be utilized for a more targeted yield improvement strategy of Philippine rice germplasm and development of superior high-yielding rice varieties.
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