Development of biofortified rice through mutation breeding
2019
Marfori-Nazarea, C.M. | Amparado, A. | Inabangan-Asilo, M.A. | Angeles, N. | Maghirang, E. | Tesoro, F.F. | Swamy, B.P.M. | Reinke, R.
Biofortification of rice with enhanced levels of Zinc (Zn) in its polished form may be a cost-effective and sustainable solution to assist in combating Zn malnutrition. The main purpose of mutation is to induce useful genetic variations in a short period of time and develop varieties with useful agronomic and nutritional traits. In this study, nine popular rice varieties: IR64, IRRI154, BR29, IRRI195, IR07F290. Cihering, IR87707-446-B-B, IRRI123, IRRI156 and two Zn donor lines: Kaliboro and Jamir were subjected to 0.8 and 0.1% Ethyl Methyl Sulfonate (EMS) pre-treatment as a mutagen. Out of 11 populations, a total of 16,298 were transplanted and 1,084 individual M1 plants were harvested. The seedlings from M2 to M4 were advanced under irrigated. Zn-deficient and saline soil conditions. EMS-treated plants showed differences in terms of morphological characteristics among its sister lines in the same population. Selections were carried out from M2 to M5 generation based on the desirable agronomic, yield and yield component traits. The M5 seedlings coming from the stressed and normal condition were transplanted in irrigated plots. Based on the grain Zn content measured using X-ray fluorescence (XRF), lines with high Zn content will be forwarded. M6 will be planted in 2019 WS in replicated trail and grain Zn will be further analyzed. This study aims to incorporate Zn biofortification in rice through mutation breeding.
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