Preliminary investigation into the ecology of marine viruses in Malaysia
2011
Heng, Pei Li | Lee, Choon Weng
In this study, we investigated the abundance of marine viruses in the coastal waters of Peninsular Malaysia. Virus counts were measured via epifluorescence microscopy. Samples were collected from Port Klang and Port Dickson, where Port Klang was eutrophic and Port Dickson was oligotrophic. Three different preservation methods were used for our samples i.e. unpreserved, preserved with formaldehyde and frozen without preservation, Based on the counts obtained, frozen samples consistently gave the lowest virus counts (F=13.65, q =7,28, P < 0.005). The counts also differed significantly between both sites with higher counts at Port Klang, which ranged from 6,68 x 10⁶ to 5.45 x10⁷ cells/mL whereas at Port Dickson, it ranged from 2,52 x 10⁶ to 1.33 x 10⁷ cells/mL (t-Test: t =2.07, df =22, P < 0.01). When comparing with bacterial abundance and Chlorophyll a concentration, the virus count was found to be significantly correlated only to Chlorophyll a concentration (r²= 0.423, df = 10, p< 0.05).
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