Changes in Benzoxazinoid dimboa levels of several native corn genotypes during vegetative-stage infestation of the Asian corn borer
2018
Caasi-Lit, M.T. | Salazar, A.M. | Purificacion, M.V. | Cuizon, R. | Macasaet, J.P.A. | Novio, B.A.
Maize synthesizes defence-related metabolites which help lessen insect-feeding damage up to vegetative stage. Changes in 2, 4-dihydroxy, 7-methoxy 1-4 (2H)-benzoxazin-3-one (DIMBOA) were analyzed in six native varieties after artificial infestation of first-brood neonate Asian corn borer (ACB), Ostrinia furnacalis (Guenee), Leaf tissues (-0.05 g) from the seedlings to early vegetative stage were excised at 0, 2, 14 and 16 days after infestation (DAI) and analyzed using High-Performance Liquid Chromatography to determine DIMBOA concentrations. This was done simultaneously with observations of leaf damage ratings differed significantly at all sampling maize stages. All native corn varieties and the positive check, MON810, showed increased production of DIMBOA production. In contrast, negative check, IPB Var 11, showed decreased DIMBOA production. In addition, leaf tissue damage rating showed higher variability from 4-16 DAI (%CV = 16.14 17.98). On the other hand, data for DIMBOA levels from Day 0 to Day 16 also showed increasing coefficient of variation (3.78 9.10). The possible defensive role of DIMBOA in the native varieties of corn against ACB is discussed.
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