Fitohemijske i funkcionalne karakteristike praškastih formi klijanaca pšenice, ovsa i ječma / Phytochemical and functional properties of wheat, oats and barley powdered sprouts

This study was performed to evaluate the phytochemical composition, and in vitro antioksidativnidant capacity, reducingpower, antihyperglycemic, antiinflammatory activities, andsimulated gastrointestinal digestion of seven-day old cerealsprouts (CS): Cultivars, barley NS565 (BSNS), barley Golozrni (BSG), wheat Spelta (WSSPE), wheat Simonida (WSSIM),oat Golozrni (OSG) and oat Jadar (OSJ). Phenolic compoundswere the most dominant bioactives in all CS. BSNS expressedsignificantly higher (p ≤ 0.05) content of total phenols, chlorophyll and carotenoids. The total flavonoids content (TFC) inCS showed that the BSNS and BSG had the higher valuerespectively. The lowest TFC was registered in OSJ (P ≤ 0.05).The FDS extracts were screened for possible antioxidant capacities using DPPH, ABTS, and reducing power (Rp) assays.The results indicated that the BSNS possessed higher antioxidant capacities in DPPH and ABTS assays, and reducingpower (IC50DPPH = 0.54 mg/ ml; IC50ABTS = 0.79 mg/ml; IC0.5RP= 9.35 mg/ml) respectively. The inhibitory effect of FDSextracts on α-glucosidase activity was investigated. The BSNSextracts exhibited higher inhibitory activity (IC50AHgA = 1.43mg/ml) against α-glucosidase (p ≤ 0.05). The antiinflammatoryactivity (Denaturation of protein in vitro) showed significantlydifferent between the CS, and Diclofenac sodium (DS). TheIC50AIA of DS and BSNS was 0.79 and 1.86 (mg/ml) respectively. The in vitro simulation of gastro-intestinal (GI) di-VIgestion showed TPC was a higher release (p ≤ 0.05) of phenolic compounds in intestinal fluid than in gastric fluid in allFDS. There was a strong positive correlation between TPC andantioxidant activities and reducing power, and also betweenTFC and antiinflammatory activity.