The isolation, purification, and characterization of kefiran exopolysaccharide from kefir granule
2017
Inal, M.
Kefiran is a polysaccharide existed in the structure of the kefir granule. It is produced by some bacteria in the granule. This exopolysaccharide, which is also secreted to kefir drink during the fermentation, has attracted due to its positive health effects and functional properties.In this study, the biomass of commercial lyophilized kefir grain was increased under laboratory conditions and the exopolysaccharide in the kefir grain was purified and characterized. Lyophilization was applied to gel form of kefiran for the experimental studies. The sugar composition of kefiran, the amount of total EPS and the qualitative sugar analysis were determined by high performance liquid chromatography, fenol-sulfuric acid method, and thin layer chromatography, respectively. The presence of functional groups was determined by Fourier transform infrared spectroscopy. Microstructure and surface morphology kefiran was analysed by scanning electron microscopy.The composition of glucose and galactose was in ratio of 1:0.3. FTIR analysis confirmed the carboxyl, hydroxyl and carbonyl functional groups presented in the EPS structure. The total amount of EPS was determined as 93%. SEM images of this exopolysaccharide showed smooth surfaces with pores. Rheological and textural profile analysis applied to kefiran gel have determined flow behavior of product. According to this, kefiran showed non-Newtonian flow behavior.
显示更多 [+] 显示较少 [-]