Enzymatic hydrolysis of sugarcane bagasse pretreated with acid or alkali
2011
Pietrobon, Vivian Cristina(Universidade de São Paulo Centro de Energia Nuclear na Agricultura Laboratório de Ecologia Aplicada) | Monteiro, Regina Teresa Rosim(Universidade de São Paulo Centro de Energia Nuclear na Agricultura Laboratório de Ecologia Aplicada) | Pompeu, Georgia Bertoni(Universidade de São Paulo Centro de Energia Nuclear na Agricultura Laboratório de Ecologia Aplicada) | Borges, Eduardo Poggi e(Fermentec Ltda) | Lopes, Mario Lucio(Fermentec Ltda) | Amorim, Henrique Vianna de(Fermentec Ltda) | Cruz, Sandra Helena da(Universidade de São Paulo Escola Superior de Agricultura Luiz de Queiroz Setor de Açúcar e Álcool) | Viégas, Ellen Karine Diniz(Universidade de São Paulo Escola Superior de Agricultura Luiz de Queiroz Setor de Açúcar e Álcool)
The aim of this study was to evaluate the performance of enzymatic hydrolysis of acid or alkali pretreated sugarcane bagasse for the production of fermentable sugars. The first step consisted of selection of commercial enzymes presenting the highest cellulolytic activities. After selection of four enzymes: HPL, CL, P1 and P4, their performances were tested in the bagasse pretreated with acid and alkali. The sugar content of the hydrolysates was analyzed by anion exchange liquid chromatography. Data showed that the joint action of 0.5% acid pretreatment, 121ºC, 30 minutes and enzyme CL provides the best results, 67.25 g of hexose and 148.13g of pentose per kg of dry bagasse.
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