IMPROVEMENT OF IN VITRO ROOTING OF MICROCUTTINGS SOME ROOTSTOCKS OF FRUIT CROPS
2022
Suleimanova, Sevil
The objects of the study were microcuttings of rootstocks of fruit crops Myrobalan 29C, MaxMa 14, Garnem 15, GF 677 and OHF 87, as well as MS and DKW nutrient media modified with various combinations and concentrations of auxins and cytokinins (IBA, NAA, BAP) in 5 variants. It was found that the optimal medium for rooting micro plants of MaxMa 14 rootstock was DKW medium with 1.0 mg/l IBA and 0.01 mg/l NAA (rooting - 82%, average number of roots - 4.2 pcs, average root length - 4.3 cm). Optimal rooting of micro plants of the rootstock Myrobalan 29C (rooting - 80.0%, average number of roots - 4.0 pcs, average length of roots - 3.5 cm), Garnem 15 (rooting - 88.0%, average number of roots - 3, 7 pcs, average root length - 2.9 cm), GF 677 (rooting - 80.0%, average number of roots - 4.7 pcs, average root length - 3.2 cm), and OHF 87 (rooting - 80, 0%, the average number of roots is 6.5 pcs, the average length of the roots is 2.9 cm) was noted on MS medium using IBA at a concentration of 1.0 mg/l and NAA at a concentration of 0.1 mg/l. In addition, there was no significant change in the efficiency of rootstock rooting of fruit crops under in vitro conditions, depending on the passage.
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