In-vitro effects of biochemical factors on trypsin activity from intestine and pyloric caeca of common kilka (Clupeonella cultriventris caspia) for inhibition of belly bursting
2011
A. Zamani | M. Rezaei email | R. Madani
The in-vitro effects of biochemical factors on trypsin activity from intestine and pyloriccaeca of common kilka (Clupeonella cultriventris caspia) was evaluated. Trypsin is known tocause belly bursting in common kilka. The assessments showed that in a range of 4-70°C,trypsin from pyloric caeca and intestine of common kilka had the maximum activity and thesatiability at 60 and 55°C, respectively. The pH assessments indicated that maximum activityand stability for trypsin were at 8.5 at pH range of 4-11. The effects of metal ions on trypsinactivity revealed that CaCl2, MgCl2 and MnCl2 increased trypsin activity while CuCl2 ØZnCl2and Al2(SO4)3 decreased its activity. The effect of inhibitors on trypsin activity also showedthat SBTI and TLCK (specific inhibitors for trypsin) significantly inhibited trypsin activity.This study suggests that belly bursting in common kilka can be prevented by trypsininactivation through application of low temperature (4°C), acidic pH, metals of CuCl2, ZnCl2and Al2(SO4)3 and inhibitors of SBTI and TLCK.
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