Simplified HPLC-UV method for the determination of α-tocopherol in plasma
2010
Alberto Bonomi | Afro Quarantelli | Carla Quarantelli | Federico Righi | Marco Renzi
Vitamin E, known for its great nutritional importance, is normally included in animal diets as DL-α-tocopherol acetate.<br />The authors propose a method that makes it possible to determine the concentration of vitamin E in plasma without<br />saponification. This method enable to avoid aggressive treatments on the analyte and complex procedures; it detects<br />vitamin E only in form of DL-α-tocopherol.<br />Lipoproteins of analysed plasma were denaturised by methanol. Vitamin E was extracted by petroleum ether in presence<br />of NaCl. The extract was dried by rotavapor at 45 °C, solubilized by methanol and injected in HPLC (C18 column, reversed<br />phase). The quantitative determination was carried out by UV detector settled on 294 nm.<br />Tests of repeatability inter-analysis and intra-analysis gave coefficient of variability (CV%) respectively of 1.64 and<br />2.41%. The mean recovery was 100%.
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