Membrane Association and Topology of Citrus Leprosis Virus C2 Movement and Capsid Proteins
2021
Mikhail Oliveira Leastro | Juliana Freitas-Astúa | Elliot Watanabe Kitajima | Vicente Pallás | Jesús Á. Sánchez-Navarro
Although citrus leprosis disease has been known for more than a hundred years, one of its causal agents, citrus leprosis virus C2 (CiLV-C2), is poorly characterized. This study described the association of CiLV-C2 movement protein (MP) and capsid protein (p29) with biological membranes. Our findings obtained by computer predictions, chemical treatments after membrane fractionation, and biomolecular fluorescence complementation assays revealed that p29 is peripherally associated, while the MP is integrally bound to the cell membranes. Topological analyses revealed that both the p29 and MP expose their N- and C-termini to the cell cytoplasmic compartment. The implications of these results in the intracellular movement of the virus were discussed.
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