Mycoplasma spp. noteikšanas metodikas izstrāde piena lopkopībā
2021
Kovalenko, K., Latvia Univ. of Life Sciences and Technologies, Jelgava (Latvia). Faculty of Veterinary Medicine | Malniece, A., Latvia Univ. of Life Sciences and Technologies, Jelgava (Latvia). Faculty of Veterinary Medicine | Terentjeva, M., Latvia Univ. of Life Sciences and Technologies, Jelgava (Latvia). Faculty of Veterinary Medicine | Titmane, L., Latvia Univ. of Life Sciences and Technologies, Jelgava (Latvia). Faculty of Veterinary Medicine | Vanaga, A., Latvia Univ. of Life Sciences and Technologies, Jelgava (Latvia). Faculty of Veterinary Medicine | Zirnitis, A., Latvia Univ. of Life Sciences and Technologies, Jelgava (Latvia). Faculty of Veterinary Medicine
The aim of the research is to develop a methodology for sampling and sample testing for the future for use in clinical practice in the dairy sector Mycoplasma spp. to determine. The selection of farms for the study was done on a voluntary basis based on the service provider information provided by a veterinarian regarding animal health problems on the holding which are associated with mycoplasmosis, such as high somatic cell counts, clinical manifestations of respiratory infections, inflammation of the joints not related to abnormalities in well-being, etc. c., or on the basis of the above laboratory-confirmed mycoplasmosis on the holding during the last year. Based on these methods, 10 dairy farms of different sizes from four regions of Latvia have been selected in the study, thus ensuring the widest possible geographical representation of isolates. Use of a pharyingo-esophageal probe to obtain a pharyngeal sample for Mycoplasma spp. to determine is easy to use compared to other airway sampling methods with to isolate mycoplasmas. The size of the sample obtained by the Pharyingo-esophageal probe is larger than in others methods to facilitate sample preparation for testing and isolation of mycoplasmas.
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