Establishment of a cell line from Chinese soft-shelled turtle Pelodiscus sinensis with the practicability of transfection and viral replication
2013
Fu, J.P. ((Chinese Academy of Sciences, Wuhan, Hubei (China). Institute of Hydrobiology, State Key Laboratory of Freshwater Ecology and Biotechnology), (University of Chinese Academy of Sciences, Beijing (China))) | Luo, Z. | Yan, Y. | Zou, P.F. | Zhang, S.H. | Qin, Q.W. | Nie, P.
A continuous cell line derived from extracranial carotid artery of the Chinese soft-shelled turtle Pelodiscus sinensis was established and characterized. It was named as STA (soft-shelled turtle artery) cell line. The cells were muscle-cell like, and were stained with antibody against smooth muscle myofilament marker, alpha-smooth muscle actin. These cells had a normal diploid chromosome number of 2n = 66, and their 12S rRNA and 16S rRNA sequences shared 99% identity with ones of the turtle in database. The cell line can be cultured well in media DMEM/F12 or M199 supplemented with 10% FBS at 28degC. It was further demonstrated that the cells were transfected successfully with pTurbo plasmid, with a transfection efficiency being over 30%. The soft-shelled turtle iridovirus (STIV) propagated in the cell line, causing typical CPE with the formation of inclusion bodies. It is suggested that the established STA cell line provides a convenient platform for studying the pathogenesis of STIV and biological aspects of the turtle.
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