Isolation and characterisation of NBS-LRR motif based resistance genes in rice
2011
Kalaivani Nadarajah | Ong, Shin Tze
The presence of a single resistance (R) gene allele can determine plant disease resistance. The protein products of such genes may act as receptors that specifically interact with pathogen-derived factors. Most functionally defined R-genes are of the nucleotide binding site-leucine rich repeat (NBS-LRR) supergene family and are present as large multigene families. The specificity of R-gene interactions together with the robustness of plant-pathogen interactions raises the question of their gene number and diversity in the genome. Genomic sequences from rice showing significant homology to genes conferring race specific resistance to pathogens were identified by systematically "scanning" the genome using a variety of primer pairs based on ubiquitous NBS motifs. Over 70 sequences were isolated and 10% are putative pseudogenes. Phylogenetic clustering of R-gene homologues within rice and other graminaceae family members was observed but not with R gene homologues from Arabidopsis thaliana. Our data indicates remarkably rapid evolution of R-gene homologues during diversification of plant families.
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