A cryopreservation method of Scizophyllum commune local strains
2011
Irnada Syuhaidah Mohd Kamal | Noraswati Mohd Nor Rashid | Maizirwan Mel
Schizophyllan commune isolates were collected from rubber plantations in Selangor and Perak area in Malaysia. Spawns were prepared using three different grains (wheat, corn and paddy). The grains were incubated for 7 days at room temperature. After the grains were fully covered with mycelia, they were place into a cryo-vial with three different treatments, 10% glycerol, sterile distilled water and no cryoprotectants. All of the treatments were frozen in liquid nitrogen for 7 days followed by thawing (cooling) at 30°C for 30 min. The grains were inoculated onto PDA agar to observe the viability (rate of survival) of the seeds. The radius of each grain with different treatments was measured. Results showed that all treatments showed 100% viability after 48 hours and wheat with no cryoprotectants showed the highest growth rate. This indicated that the grains have mycelial protective properties. In this study, a cryopreservation process was simplified as opposed to the conventional method in which 10% glycerol was used as the cryoprotectant. This study promotes an alternative method for cryopreservation which is more economical as no cryoprotectants were required.
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